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首页 经销厂牌 Zedira Protein Labeling

Protein Labeling

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Protein labeling using transglutaminase (TGase) is a smart enzymatic labeling alternative to chemical protein labeling procedures. 

Protein biotinylationPEGylation or site-directed protein labeling with fluorescent dyes are common applications of TGase labeling.

Zedira’s protein labeling tools are available as
  • TGase Protein Labeling Kits
  • Individual components
  • TGase Labeling Service




Advantages of transglutaminase (TGase) mediated site-specific protein labeling: 

  • Defined degree of labeling
  • Defined label position(s)
  • Homogenously labeled protein
  • Higher solubility in water
  • Minimized amounts of unlabeled protein
  • No or reduced impact on bioactivites of labeled proteins


Principle of TGase-catalyzed protein labeling, protein modification and protein conjugation

TGases catalyse the acyl transfer reaction between the γ-carboxyamide group of protein-bound glutamine residues and the ε-amino group of protein-bound lysine or primary amines. Thus, primary amines as well as glutamine-containing peptides may carry a broad variety of labels like biotinPEG or fluorescent dyes etc.

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Requirements for transglutaminase labeling

Transglutaminase protein labeling requires substrate sequences on the target protein surface, which are in general not abundant on proteins:
  • Accessible lysine residues
  • or accessible glutamine residues
  • otherwise transglutaminase substrate sequence tags can be introduced recombinantly, if no lysine or glutamin residues are accessible

Examples for utilization of modified proteins

  • Fluorescence-based HTS-assay
  • Diagnostics (antibodies, antibody-bindingproteins, lectins etc.)
  • Therapeutic proteins (PEGylation)
  • Protein immobilization (after biotinylation)


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