精磊 Mouse Model for Cerebral Ischemia (CI)
学术研究
~~~高浓度EGFP抗体~~~NQBB血清+生长因子--效果奇佳~~~USCN ELISA Kit~~~Creative Biolabs抗体~~~治疗用抗体~~~核酸萃取仪~~~各式仪器~~~Bilfil各式耗材~~~
首页 经销厂牌 USCN Animal models 【USCN】 Mouse Model for Cerebral Ischemia (CI)

【USCN】 Mouse Model for Cerebral Ischemia (CI)

相关连结 Click here

Brain Ischemia; Cerebrovascular Ischemia; MCAO

Product No.                    DSI523Mu01
Organism Species          Mus musculus (Mouse)     Same name, Different species.
Prototype Species          Human
Source                             Ischemia-Reperfusion with MCAO
Model Animal Strains   Balb/c Mice(SPF level), male, healthy, body weight 25g~30g
Modeling Grouping      Randomly divided into groups: Control group, Model group, Positive drug group and Test drug group, 15 mice per group.
Modeling Period            24 hours, 3 days or 7 days
Modeling Method
1. 3% sodium pentobarbital (80 mg/kg), neck skin preparation, disinfection, insert the anus temperature probe, maintain the temperature within 37±0.5.
2. Incision of the middle of the neck, exposure the right common carotid artery, internal carotid artery and external carotid artery. Using 7-0 silk (at 2mm distance from the common carotid artery bifurcation ) to ligate the external carotid artery at the far end of the heart, pierces another 7-0 silk in the external carotid artery , and hit a slipknot near the bifurcation of the common carotid artery.
3. Use arterial clamps to clamp the common carotid artery. Make a small cut on the external carotid artery (1.5 mm distance from bifurcation of common carotid artery ), a root tip treated 0.18 mm diameter nylon line from the cut insertion into the internal carotid artery, and inward into the middle cerebral artery, nylon line insertion depth distance carotid artery bifurcation about 9±1mm.
4. Ischemia 90 mins and pull the suture, a 7-0 silk ligates artery proximal, 5-0 silk suture wounds on the neck, povidone iodine disinfection wound, put mice in a heating pad, and feed on constant temperature raising box after mice awaked.
5. 24hrs after operation, neurological function score is evaluated, and then the mice are injected intraperitoneally with 3% sodium pentobarbital, and the brains are stained with TTC and pathological staining.
Applications         Diesase model
Download             n/a
UOM                    Each case

0af52f22286a08c13130d4a479bab1e5.png7d57bc2de56a5d4b16f1edfd2a7454ef.jpg


Model Evaluation of the Mouse Model for Cerebral Ischemia (CI)

1.Neurological dysfunction score 
Longa and Bederson's 5 point systems,make a score on mice awaked from anaesthesia 24 hours later. 
0 points: no nerve injury symptoms
1 points can not fully extend the forepaw
2 points: to the opposite side
3 points: to the side dump
4 points: can not walk spontaneously, loss of consciousness
2.TTC staining
Take the brain and store at -20℃, 1% TTC (W/V), 37℃ water bath for TTC dissolved, the frozen brain tissue slices, placed in 10ml TTC solution, 37℃, 10min. Normal brain tissue staining is bright red, and the infarct area is pale.


Pathological Results of the Mouse Model for Cerebral Ischemia (CI)

Take the brain, 4% poly formaldehyde solution fixed, after dehydration of sucrose solution, the OCT embedded to make the frozen section (slice 10um), Nissl staining and the staining resluts are used for the evaluation of infarct size.


Cytokines Level of the Mouse Model for Cerebral Ischemia (CI)


Statistical Analysis of the Mouse Model for Cerebral Ischemia (CI)

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±s), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.


Related products

Catalog No.Organism species: Mus musculus (Mouse)Applications (RESEARCH USE ONLY!)
DSI523Mu01Mouse Model for Cerebral Ischemia (CI)Diesase model
DSI523Mu02Mouse Model for Cerebral Ischemia (CI)n/a
DSI523Mu03Mouse Model for Cerebral Ischemia (CI)n/a


订阅商品讯息订阅商品讯息 Copyright © 2016 GenLabBiotech Co.,Ltd. All rights reserved.
Powered by www.url.com.tw