USCN ELISA Kit for Immunoglobulin G (IgG)
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首页 经销厂牌 USCN 【USCN】 ELISA Kit for Immunoglobulin G (IgG)

【USCN】 ELISA Kit for Immunoglobulin G (IgG)

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Organism SpeciesChicken (Gallus)





















Product No.                CEA544Ga

Organism Species      Chicken (Gallus)Same name, Different species.

Test Method               Competitive Inhibition

Assay Length              2h

Detection Range         1.23-100ug/mL

Sensitivity                   The minimum detectable dose of this kit is typically less than 0.55ug/mL.

Sample Type               serum, plasma and other biological fluids

Download                    Instruction Manual

UOM                           48T   96T    96T*5   96T*10   96T*100


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Specificity of the ELISA Kit for Immunoglobulin G (IgG)

This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG).
No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.


Recovery of the ELISA Kit for Immunoglobulin G (IgG)

Matrices listed below were spiked with certain level of recombinantImmunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G (IgG) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)97-105101
EDTA plasma(n=5)89-9693
heparin plasma(n=5)98-105101


Precision of the ELISA Kit for Immunoglobulin G (IgG)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate. 
CV(%) = SD/meanX100 
Intra-Assay: CV<10% 
Inter-Assay: CV<12% 


Linearity of the ELISA Kit for Immunoglobulin G (IgG)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-94%93-102%88-104%86-103%
EDTA plasma(n=5)99-105%97-105%85-96%81-98%
heparin plasma(n=5)94-103%81-98%82-95%91-99%


Stability of the ELISA Kit for Immunoglobulin G (IgG)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.


Assay procedure summary of the ELISA Kit for Immunoglobulin G (IgG)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.


Test principle of the ELISA Kit for Immunoglobulin G (IgG)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin G (IgG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample.


Related products

Catalog No.Organism species: Chicken (Gallus)Applications (RESEARCH USE ONLY!)
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