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首页 经销厂牌 USCN ELISA Kit 【USCN】 ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

【USCN】 ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

相关连结 Click here

CSF2; GM-CSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor




















Product No.             SEA045Ra

Organism Species   Rattus norvegicus (Rat)Same name, Different species.

Test Method            Double-antibody Sandwich

Assay Length          3h

Detection Range     7.8-500pg/mL

Sensitivity               The minimum detectable dose of this kit is typically less than 3.0pg/mL.

Sample Type           serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Download                Instruction Manual

UOM                       48T   96T   96T*5   96T*10   96T*100


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Specificity of the ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF).
No significant cross-reactivity or interference between Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and analogues was observed.

Recovery of the ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Matrices listed below were spiked with certain level of recombinantColony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and the recovery rates were calculated by comparing the measured value to the expected amount of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) in samples.

Matrix

Recovery range (%)

Average(%)

serum(n=5)

80-104

90

EDTA plasma(n=5)

79-101

95

heparin plasma(n=5)

96-105

99


Precision of the ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) were tested on 3 different plates, 8 replicates in each plate. 
CV(%) = SD/meanX100 
Intra-Assay: CV<10% 
Inter-Assay: CV<12% 

Linearity of the ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

81-90%

97-105%

83-103%

94-103%

EDTA plasma(n=5)

80-102%

87-95%

90-99%

98-105%

heparin plasma(n=5)

85-95%

86-97%

79-96%

90-99%


Stability of the ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately. 

Test principle of the ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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ELISA / CLIA Experiment Service
96-well Plate Assay Data Analysis Software
Real Time PCR Experimental Service

INCREMENT SERVICES

Single-component Reagents of Assay Kit
Lysis Buffer Specific for ELISA / CLIA
ELISA Kit Customized Service
Disease Model Customized Service
Serums Customized Service
TGFB1 Activation Reagent


Related products

Catalog No.

Organism species: Rattus norvegicus (Rat)

Applications (RESEARCH USE ONLY!)

APA045Ra01

Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Cell culture; Activity Assays.

RPA045Ra01

Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Positive Control; Immunogen; SDS-PAGE; WB.

PAA045Ra01

Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

WB; IHC; ICC; IP.

MAA045Ra21

Monoclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

WB; IHC; ICC; IP.

SEA045Ra

ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Enzyme-linked immunosorbent assay for Antigen Detection.

SCA045Ra

CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Chemiluminescent immunoassay for Antigen Detection.

 


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