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MMLV High Performance Reverse Transcriptase

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Applications

  • First-strand cDNA synthesis for subsequent PCR or real-time PCR.
  • RT-PCR validation of gene expression data obtained from microarray experiments.
  • RT-PCR validation and quantification of gene silencing by RNA interference.

The MMLV High Performance Reverse Transcriptase (MMLV HP RT) and the MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit both produce full-length first-strand cDNA from total cellular RNA preparations or purified poly(A) RNA.

MMLV HP RT demonstrates significantly greater reverse transcriptase activity than other commercially available MMLV RT enzymes. Typically, just 100 units of MMLV HP RT are required for full-length cDNA synthesis compared to 200 units of competitive MMLV RT enzymes. MMLV HP RT includes a 10X Reaction Buffer, optimized for synthesis of full-length cDNA from long RNA templates, and DTT.


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