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首页 经销厂牌 AMPLIQON 2x Taq Master Mix, Red 耐热聚合酶预混组

2x Taq Master Mix, Red 耐热聚合酶预混组

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安普康序列扩增之聚合酶及主混合反应试剂 Ampliqon 2x Taq Master Mix RED,1.25ml
























Features: 
  • Premixed solution
  • Minimal optimisation
  • Direct loading onto agarose and SDS DNA gels
  • Easy visualisation of pipetting
  • Dye front runs at 300 - 1000 bp on 0.5 - 1.5 % agarose gel
  • No need for a separate loading dye
  • Increased reproducibility
  • High product yield
  • dUTP incorporation possible
  • Processes up to 5 kb
  • Leaves a 3'dA overhang
  • No need for separate loading dye
  • Taq DNA Polymerase Master Mix Red is ready-made for electrophoresis and visualization
  • Fewer handling steps
  • 操作简单 反应体积为20 µl

COMPARISON BETWEEN TAQ DNA POLYMERASE MASTER MIX RED AND TWO COMPETITORS
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Ampliqon Taq DNA Polymerase Master Mix RED was compared with a Taq DNA Polymerase master mixes from two well recognised competitors. Four different DNA target varying in length PAH (203 bp), Q8 (727 bp), CFTR (613 bp) and BAIP (788) were evaluated. Ampliqon Taq DNA Polymerase Master Mix RED performed equally well or better in this study. Each Amplification has been conducted according to suppliers manual.

RED DYE EXHIBITS NO PCR INTERFERENCE
79d1db6eb15eac2905f4d57d4f846b65.jpg
The red dye does not interfere with PCR performance. Two different DNA targets; PAH (203 bp) and BAIP (788 bp) were amplified in duplicates using Taq Master Mix RED and visualised on a agarose gel.

PRODUCT INFO

Taq DNA Polymerase Master Mix RED

1.1x and 1.5 MgCl2 
1.1x and 2.0 mM MgCl2 
2x and 1.5 mM MgCl2 
2x and 2.0 mM MgCl2 


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