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首页 经销厂牌 AMPLIQON Ammonium Buffer

Ammonium Buffer

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Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or th





















FEATURES

  • Promotes amplification on most DNA templates
  • High yield
  • High specificity
  • Tolerance for primer annealing temperature
  • 10x Buffer


AVAILABLE IN 4 FORMULATIONS:

  • 15 mM MgCl2,
  • Mg2+ free
  • Tween free
  • Mg2+ + Tween free


DESCRIPTION

Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg2+ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences. 


TIP - CHOOSE THE RIGHT BUFFER

Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM


Ammonium Buffer: Minimal need for optimisation.
A broad range of Mg2+ concentrations and temperatures results in specific products with high yield (Lanes 1.5 - 2.5 and lanes 57-66)

abe17ea09ea16b2b36ae1aadb221b217.jpg


Standard Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products. (Lanes 1.5 - 2.0 and lanes 60-66)

4d4d366c902b0541c8198defed933f5e.jpg


Combination Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products with high yields. (Lane 1.5 and lanes 60-66)

1a71c52188726492ca45cee642dc1f73.jpg



Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg2+ concentrations or temperatures. The first part shows a Mg2+ dilution series from 0.5 - 4.5 mM MgCl2, at 60°C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl2. M: marker.

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PRODUCT INFO

Ammonium Buffer

15 mM MgCl2 
Mg2+ free 
Tween free 
Mg2+ free, Tween free 


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