学术研究
感谢客户的支持 →→原价11450元的western blot tool box,现在超杀特价只要5000元~~要抢要快~~
首页 经销厂牌 AMPLIQON RealQ Plus Master Mix Green

RealQ Plus Master Mix Green

相关连结 Click here

RealQ Plus Master Mix Green enables real-time-based DNA amplification with high specificity and efficiency. Real-time PCR is a sensitive and reliable method for gene a





















FEATURES

  • Reliable master mix for real-time PCR
  • Based on DNA-binding fluorescent dye detection
  • Reliable quantification
  • High specificity, stability, reproducibility and efficiency
  • Reaction set-up at room temperature
  • Premixed all-in-one 2x solution


DESCRIPTION

RealQ Plus 2x Master Mix Green is composed of TEMPase Hot Start DNA Polymerase, dNTPs, fluorescent dye and an optimised buffer system. Furthermore, this master mix is available with high, low or without ROXTM ensuring optimal performance on most of the commonly used real-time PCR instruments. Just add DNA template and primers.

When the fluorescent Green dye is free in the solution, it emits a very low fluorescent signal. As soon as the dye binds to the double-stranded DNA the signal increases significantly (thousand fold), which makes the fluorescent signal of the dye directly proportional to the amount of amplified dsDNA.


ROX SELECTION GUIDE

Use this guide to select the correct level of ROX™ reference dye for your real-time PCR application.

 

HIGH EFFICIENCY AND SPECIFICITY 

9ac710296c64430bf13e9a2a114d88e7.png72e9cf9d9bdb179db77dfea294073143.pngcd36fe1b843a2b454bd73ed9e6007467.png
To examine the efficiency of the RealQ Plus 2x Master Mix Green, High ROXTM, a 4-fold dilution series with human gDNA was set up for PAH target (203 bp). Samples were made in triplicates starting with 80 ng down to 80 pg gDNA per well. The results depicted here shows high precision and efficiencies close to 100 %. The identical melt curves show a high specificity of the product and the standard curve shows linear detection range and a high accuracy of the TEMPase Hot Start DNA Polymerase


REPRODUCIBILITY
0f96c09c71220566b340211b15ca7ad7.png
80 replicates of RealQ Plus 2x Master Mix Green, High ROX™ and 20 ng gDNA, show a standard deviation of only 0.084.

STABILITY AT ROOM TEMPERATURE
b135f7a8cab353a39a591f9d7758a0c5.png34aa0315a4461b33968f4691f5870838.png
2e8a87684bd54152e4023f0f38ef2f28.png7c1a4d33ec40198f4d60e58f46c782c8.png
Two plates were pre-assembled for real-time PCR reaction and incubated in darkness at room temperature for 48 and 72 hours. The results show high stability and complete inactivation of the TEMPase before hot start. It allows the scientist to set up the reaction and run the plate several hours later, when convenient.


PRODUCT INFO

RealQ Plus Master Mix Green

Green without ROX 
Green with low ROX 
Green with high ROX 


Copyright © 2016 GenLabBiotech Co.,Ltd. All rights reserved.
Powered by www.url.com.tw