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USCNK ELISA Kit for Interleukin 8 (IL8)

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CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1





















Product No.               SEA080Ca

Organism Species     Canis familiaris; Canine (Dog)       Same name, Different species.

Test Method              Double-antibody Sandwich

Assay Length            3h

Detection Range       0.156-10ng/mL

Sensitivity                 The minimum detectable dose of this kit is typically less than 0.057ng/mL.

Sample Type             serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Download                  Instruction Manual

UOM                         48T   96T   96T*5   96T*10   96T*100


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Specificity of the ELISA Kit for Interleukin 8 (IL8)

This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8).
No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.


Recovery of the ELISA Kit for Interleukin 8 (IL8)

Matrices listed below were spiked with certain level of recombinantInterleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 8 (IL8) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)85-10396
EDTA plasma(n=5)87-9491
heparin plasma(n=5)97-105101


Precision of the ELISA Kit for Interleukin 8 (IL8)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate. 
CV(%) = SD/meanX100 
Intra-Assay: CV<10% 
Inter-Assay: CV<12% 


Linearity of the ELISA Kit for Interleukin 8 (IL8)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-101%88-102%99-105%94-103%
EDTA plasma(n=5)91-105%85-101%88-95%92-101%
heparin plasma(n=5)86-101%89-99%94-101%81-101%


Stability of the ELISA Kit for Interleukin 8 (IL8)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.


Assay procedure summary of the ELISA Kit for Interleukin 8 (IL8)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately. 


Test principle of the ELISA Kit for Interleukin 8 (IL8)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 (IL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


Related products

Catalog No.Organism species: Canis familiaris; Canine (Dog)Applications (RESEARCH USE ONLY!)
RPA080Ca01Recombinant Interleukin 8 (IL8)Positive Control; Immunogen; SDS-PAGE; WB.
PAA080Ca01Polyclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC; IP.
LAA080Ca71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC.
LAA080Ca81FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC; IF.
MAA080Ca22Monoclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC; IP.
SEA080CaELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection.
IEA080CaInstant ELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA080CaCLIA Kit for Interleukin 8 (IL8)Chemiluminescent immunoassay for Antigen Detection.
KSA080Ca01ELISA Kit DIY Materials for Interleukin 8 (IL8)Main materials for "Do It (ELISA Kit) Yourself".


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