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首页 经销厂牌 USCNK ELISA Kit USCNK ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

USCNK ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

相关连结 Click here

VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor





















Product No.               SEA143Ca

Organism Species     Canis familiaris; Canine (Dog)     Same name, Different species.

Test Method              Double-antibody Sandwich

Assay Length            3h

Detection Range       15.6-1,000pg/mL

Sensitivity                 The minimum detectable dose of this kit is typically less than 5.6pg/mL.

Sample Type             Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Download                  Instruction Manual

UOM                         48T   96T   96T*5   96T*10   96T*100


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Specificity of the ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA).
No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.


Recovery of the ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

Matrices listed below were spiked with certain level of recombinantVascular Endothelial Growth Factor A (VEGFA) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Endothelial Growth Factor A (VEGFA) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)88-9592
EDTA plasma(n=5)83-9388
heparin plasma(n=5)88-10291


Precision of the ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate. 
CV(%) = SD/meanX100 
Intra-Assay: CV<10% 
Inter-Assay: CV<12% 


Linearity of the ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vascular Endothelial Growth Factor A (VEGFA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)79-102%79-103%94-103%79-97%
EDTA plasma(n=5)89-98%93-104%90-98%83-98%
heparin plasma(n=5)84-91%78-88%78-98%87-105%


Stability of the ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.


Assay procedure summary of the ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately. 


Test principle of the ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor A (VEGFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Endothelial Growth Factor A (VEGFA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


Related products

Catalog No.Organism species: Canis familiaris; Canine (Dog)Applications (RESEARCH USE ONLY!)
RPA143Ca01Recombinant Vascular Endothelial Growth Factor A (VEGFA)Positive Control; Immunogen; SDS-PAGE; WB.
PAA143Ca01Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)WB; IHC; ICC; IP.
LAA143Ca71Biotin-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)WB; IHC; ICC.
MAA143Ca21Monoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)WB; IHC; ICC; IP.
SEA143CaELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA143CaCLIA Kit for Vascular Endothelial Growth Factor A (VEGFA)Chemiluminescent immunoassay for Antigen Detection.


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