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首页 经销厂牌 USCNK ELISA Kit USCNK ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

USCNK ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

相关连结 Click here

CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein





















Product No.             SEA041Hu

Organism Species   Homo sapiens (Human)Same name, Different species.

Test Method            Double-antibody Sandwich

Assay Length          3h

Detection Range     15.6-1,000pg/mL

Sensitivity               The minimum detectable dose of this kit is typically less than 6.1pg/mL.

Sample Type           Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Download                Instruction Manual

UOM                       48T   96T   96T*5   96T*10   96T*100


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Specificity of the ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

This assay has high sensitivity and excellent specificity for detection of Neutrophil Activating Protein 3 (NAP3).
No significant cross-reactivity or interference between Neutrophil Activating Protein 3 (NAP3) and analogues was observed.

Recovery of the ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

Matrices listed below were spiked with certain level of recombinantNeutrophil Activating Protein 3 (NAP3) and the recovery rates were calculated by comparing the measured value to the expected amount of Neutrophil Activating Protein 3 (NAP3) in samples.

Matrix

Recovery range (%)

Average(%)

serum(n=5)

97-105

101

EDTA plasma(n=5)

83-99

90

heparin plasma(n=5)

92-101

95


Precision of the ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Activating Protein 3 (NAP3) were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Activating Protein 3 (NAP3) were tested on 3 different plates, 8 replicates in each plate. 
CV(%) = SD/meanX100 
Intra-Assay: CV<10% 
Inter-Assay: CV<12% 

Linearity of the ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neutrophil Activating Protein 3 (NAP3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-101%

90-98%

80-93%

80-99%

EDTA plasma(n=5)

82-98%

85-96%

89-96%

90-104%

heparin plasma(n=5)

89-105%

80-102%

97-105%

80-92%


Stability of the ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.


Assay procedure summary of the ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately. 

Test principle of the ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Activating Protein 3 (NAP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Activating Protein 3 (NAP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Activating Protein 3 (NAP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Activating Protein 3 (NAP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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Organism species: Homo sapiens (Human)

Applications (RESEARCH USE ONLY!)

APA041Hu01

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Polyclonal Antibody to Neutrophil Activating Protein 3 (NAP3)

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SEA041Hu

ELISA Kit for Neutrophil Activating Protein 3 (NAP3)

Enzyme-linked immunosorbent assay for Antigen Detection.

SCA041Hu

CLIA Kit for Neutrophil Activating Protein 3 (NAP3)

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KSA041Hu01

ELISA Kit DIY Materials for Neutrophil Activating Protein 3 (NAP3)

Main materials for "Do It (ELISA Kit) Yourself".

 


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